Insertion junction: CLIP2.038346_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038346
Chromosome:chromosome 17
Location:1563700
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g707150 LIC2 Hypothetical protein; (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCGCCAGAGCAGGCGGACGCGATGTCGGACATGCTGTGCTTTTGGTG
Internal bar code:CGATGCGCCCTGGGTTGGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:529
LEAP-Seq percent confirming:67.5
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGTAAGAGGGAGAGGCA
Suggested primer 2:GCGTGGACGATGAAGATTGC