Insertion junction: CLIP2.038371_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038371
Chromosome:chromosome 2
Location:6436497
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388450 ELG29 Exostosin-like glycosyltransferase 29; (1 of 2) PTHR31389:SF4 - PROTEIN F32B4.1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACATCATGAATGTAACCGCCACCCGCAAACCCGCCCCTGTCCGCTCGC
Internal bar code:CACAGCCACTCAGACGTGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2296
LEAP-Seq percent confirming:92.5
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:CACAAACAATTCGCCAGCCA
Suggested primer 2:GACAACCCAAACACTCGTGC