Insertion junction: CLIP2.038381_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.038381
Chromosome:chromosome 9
Location:2628911
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801018 (1 of 1) PTHR11390//PTHR11390:SF21 - PROKARYOTIC DNA TOPOISOMERASE // DNA TOPOISOMERASE 3-ALPHA intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGATGGGGTCACAACGCGTCTACTATGGATGCGAATTACAATTGAAGGG
Internal bar code:CTATGCGACCTTGTACCTTCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:58.8235
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGAATGGGTGGGGGTTAG
Suggested primer 2:CTCCCCTTTTCCCGTTCCTC