| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038396 |
| Chromosome: | chromosome 17 |
| Location: | 209619 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697300 | CEP16 | Cysteine endopeptidase; (1 of 1) 2.7.11.1//3.4.22.67 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Zingipain / Zingibain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATAGGCCTCAGAACAAGACAAAATTCACGCTGTATGGTCTCCCGTCGGA |
| Internal bar code: | GGGCAGCTTGCTACATTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4547 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCACTCTTGCCATCCTT |
| Suggested primer 2: | TTCCAACGAAGAGCCCAGAC |