Insertion junction: CLIP2.038406_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.038406
Chromosome:chromosome 6
Location:5281800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284450 PPP24 (1 of 2) K15731 - carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase [EC:3.1.3.16] (CTDSP); Phosphoprotein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGGGGTAACATCCTCCCCCGAAGAGAGAATTTGAGGCAACGGACTTC
Internal bar code:AACTTGGCACGGCTTTACGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:382
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTTATTCGCCGCAGCTC
Suggested primer 2:GACATGGAGGAGATGGAGCG