Insertion junction: CLIP2.038409_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.038409
Chromosome:chromosome 16
Location:6535889
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g674500 ABCA4 ABC transporter 4; (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTTTGTGACGGTGTGCGACGGCGCAGACGGTGGCACGGGCACTGGGCC
Internal bar code:GTGCGGATAAACGGTAATGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCATCTTCCTGCTGCTGTGG
Suggested primer 2:TTCGTCAAAGGTGGTCTCGG