| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.038421 |
| Chromosome: | chromosome 3 |
| Location: | 2578072 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160050 | FAP184 | (1 of 1) PTHR15654:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 96; Flagellar Associated Protein 184 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTCCTGCAAGTTGGCAGAAGGAACCGGTGCATTCAGTACCACAGTGG |
| Internal bar code: | CGGGTTACACCCCTAATGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1966 |
| LEAP-Seq percent confirming: | 94.3396 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACATATTGCTTGGCACG |
| Suggested primer 2: | GCCCCACAAGTACCGTAGTC |