| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038481 |
| Chromosome: | chromosome 14 |
| Location: | 3098240 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801647 | (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGGACGCCTCTGCATGCATAAACGTAATGCAAAGGGCCAGACTGAGC |
| Internal bar code: | CAGAACTTTGTAGGTGCAATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4422 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTCGCTGTCCCTATCCA |
| Suggested primer 2: | CTCATCGTGCGCCATAGTCT |