| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038482 |
| Chromosome: | chromosome 10 |
| Location: | 1211970 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426250 | (1 of 1) K14800 - pre-rRNA-processing protein TSR2 (TSR2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTTGTACCCGCATGGGCTCCCCTTGTGCCCTCCTCCCTCCTCCCTCC |
| Internal bar code: | CTCACGTCATTTTCGCATGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGGTAACCCCTCCCTT |
| Suggested primer 2: | ACCCCCTAATCCCTGTACCC |