| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038487 |
| Chromosome: | chromosome 6 |
| Location: | 2634299 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270200 | PLC1,PLC | Phospholipase C; (1 of 1) 3.1.4.11 - Phosphoinositide phospholipase C / Triphosphoinositide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCAGGTCAAGGAGCTGGGCGCGGACGATGCGGACGAGGAGCGGGAGA |
| Internal bar code: | AGGAACACTTGTTAAGTTAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3853 |
| LEAP-Seq percent confirming: | 93.9024 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGTGCACAGGATCGAG |
| Suggested primer 2: | GTTCAAGGTTTCCCGCACAC |