| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038513 |
| Chromosome: | chromosome 13 |
| Location: | 2362673 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579100 | APC1 | (1 of 1) K03348 - anaphase-promoting complex subunit 1 (APC1); Anaphase promoting complex subunit 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCGCGTGTGTGTGCGTGTGCGTCCAAAGGGTAAGGGCGATTCCGTGG |
| Internal bar code: | CGATGGGAAATCCGGTATACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGATACCCCCGCATTGCA |
| Suggested primer 2: | CTGCAGTTTCACCTTTGCCC |