Insertion junction: CLIP2.038513_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038513
Chromosome:chromosome 13
Location:2362675
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g579100 APC1 (1 of 1) K03348 - anaphase-promoting complex subunit 1 (APC1); Anaphase promoting complex subunit 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCGCAGGGTCTGCGTGTCCCCTATCTCGTCTTTCCTAACATACACTCG
Internal bar code:GGGTGACTTAGGGGGGACTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:622
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCAGTTTCACCTTTGCCC
Suggested primer 2:AAAGATACCCCCGCATTGCA