Insertion junction: CLIP2.038529_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.038529
Chromosome:chromosome 4
Location:1329932
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g214600 (1 of 30) 3.1.3.48 - Protein-tyrosine-phosphatase / PTPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCTACCGGTGCAGCAGCCCGGGGGATGACGTGGAGTGTGCGGCGGCC
Internal bar code:ATGTTTATCTATGGGGGGTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:288
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGATTTCGGGCAGATTTC
Suggested primer 2:GGCGTCGCTTTTTGACAACT