| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.038530 |
| Chromosome: | chromosome 3 |
| Location: | 3686985 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168650 | (1 of 1) PF08621 - RPAP1-like, N-terminal (RPAP1_N) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATAATACCTGCTGCCGGAGCAGGTCATCCTCGGAGCCAAAGTCCAGGT |
| Internal bar code: | GCCGTTTCTCCAGTAGGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2392 |
| LEAP-Seq percent confirming: | 97.4227 |
| LEAP-Seq n confirming: | 189 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 194 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACGTGTGGAATGCAAAG |
| Suggested primer 2: | AGGTCCTCCTCCGAATCCAA |