| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038531 |
| Chromosome: | chromosome 16 |
| Location: | 7543564 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683147 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | CDS | |
| Cre16.g683259 | MCP29 | (1 of 1) PTHR24089:SF270 - SOLUTE CARRIER FAMILY 25 MEMBER 48; Mitochondrial substrate carrier protein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCGTCAACAGCAGCTGGACTGCGCGCTTCGCAGAGGAGGGTGCGTGC |
| Internal bar code: | AATCAGGTCTAGGGGTAAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2576 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCAGCCCATGTGCATTA |
| Suggested primer 2: | TCAGGACAGGAAACGAAGGC |