Insertion junction: CLIP2.038545_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038545
Chromosome:chromosome 17
Location:1990531
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g710600 (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGAAGGCGGAGGCAAAGGCCGACGCGGGCTTGGGGCGCGGCCCCACCT
Internal bar code:TGGCTGAGGAAGCTTAGGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2791
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTCGACCCTCAACTCCTG
Suggested primer 2:AACGTCCCATGCAATACCGT