Insertion junction: CLIP2.038555_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038555
Chromosome:chromosome 17
Location:3244930
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g721750 ELG9 Exostosin-like glycosyltransferase 9; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTGAGCACCGGCTGGCGATCCAACCCGGCGCCTGTATGTCCAGGTCG
Internal bar code:GTGTAAACTAGTTTTGCAACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1745
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAACTCTCCCCCATCCCC
Suggested primer 2:ACGCAACAATCCCAGAGAGG