| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038557 |
| Chromosome: | chromosome 12 |
| Location: | 8115601 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552350 | (1 of 4) PF04191 - Phospholipid methyltransferase (PEMT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTTGGGGGCATGGGCATCGTATACAGCAGGTGTGTGTGTGTGTGTGT |
| Internal bar code: | CGTGGCTCAACGCCGTGGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3301 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGCCCATCTCACCATGT |
| Suggested primer 2: | CTCTTGTGTGTTGCAGTGGC |