| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.038560 |
| Chromosome: | chromosome 7 |
| Location: | 3275350 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334400 | EXN10 | Exonuclease RNase T and DNA polymerase III; (1 of 1) PTHR30231 - DNA POLYMERASE III SUBUNIT EPSILON | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACTTGAGGTTGTGCGCCACAATGACGCCGCAGTTCCAGCGCTGCAGG |
| Internal bar code: | ACGCCCAGCTGAGGATCTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1307 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGACTACGGTTTCCACGG |
| Suggested primer 2: | CTCAAGTGCTAGGCCTGCTT |