| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.038560 |
| Chromosome: | chromosome 16 |
| Location: | 1169298 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650400 | CPL20 | Hypothetical zinc-dependent DNA-binding protein; (1 of 1) IPR000104//IPR006734 - Antifreeze protein, type I // Protein of unknown function DUF597 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTGCTTGAAGTGGCTTGCATGGGAGATGGCCTGGGGCACTGTGACAC |
| Internal bar code: | CCCGGCTAGTAGGCGCTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2032 |
| LEAP-Seq percent confirming: | 6.25 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTATGTCTGTGCCGGCA |
| Suggested primer 2: | TGTACGTGAGCATTGTGCCT |