Insertion junction: CLIP2.038579_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038579
Chromosome:chromosome 1
Location:7884280
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g055440 CSR8 Carbohydrate sulfotransferase-related 8; (1 of 1) 2.8.2.30 - [Heparan sulfate]-glucosamine 3-sulfotransferase 3 / Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACACACACTACTTCAGCGGCACCGGCAGCAAGTACGACAAGAGCTCGC
Internal bar code:GCGATCTTAGATCGAGGGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3618
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGAACTCCAGGAACCGC
Suggested primer 2:AGGCACCCATCCAGAAATGG