| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.038587 |
| Chromosome: | chromosome 3 |
| Location: | 4427866 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175300 | FAS14,FLA9 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; FAS1 domain containing protein; | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCGTTGGCGCAGGCGGGCGGCGGCGGCGGTGGCGGCGGCGCGGCCCT |
| Internal bar code: | GCTTACACGTAGGCGTCCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1451 |
| LEAP-Seq percent confirming: | 58.5366 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATGTGGTGTTTGGGGT |
| Suggested primer 2: | AAGCGGAGTGTCCAGTGAAG |