| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038595 |
| Chromosome: | chromosome 17 |
| Location: | 4610315 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732350 | GOX4 | Glyoxal oxidase 4; (1 of 12) 1.1.3.9 - Galactose oxidase / Beta-galactose oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTGACGACGACGCCGTCGCTGGAGGCCAGCAGGCTGCCTTTGGAGTC |
| Internal bar code: | GAGCTCTGGGTCTGATGTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1308 |
| LEAP-Seq percent confirming: | 95.5882 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAAAGGCGTCGGTAAGG |
| Suggested primer 2: | GGTGCTGTGATGTACGTCCA |