| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038604 |
| Chromosome: | chromosome 10 |
| Location: | 5026222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454700 | (1 of 1) IPR001611//IPR003591//IPR025875 - Leucine-rich repeat // Leucine-rich repeat, typical subtype // Leucine rich repeat 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTTGTCGCCCCACGCGCCGCGGCGCCAGGCGACGGCGGCCGCGCTGC |
| Internal bar code: | TATGTGTGCGATTAAGGCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGACGAGGTAGAGCTG |
| Suggested primer 2: | CCAAACCACTGCCCACATTG |