| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038635 |
| Chromosome: | chromosome 6 |
| Location: | 7495345 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300900 | GLE1 | Nucleoporin GLE1; (1 of 1) K18723 - nucleoporin GLE1 (GLE1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAATTGAAGGGACAGCCCGACAAAGTTATGGGTGCGGATTACAAAGTCG |
| Internal bar code: | CCAGACGGGGACAAGTCTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4385 |
| LEAP-Seq percent confirming: | 82.5581 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCACCAATAGGCGAACG |
| Suggested primer 2: | AACAGTGACCCCCAAAGCAA |