| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038640 |
| Chromosome: | chromosome 16 |
| Location: | 7547391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683259 | MCP29 | (1 of 1) PTHR24089:SF270 - SOLUTE CARRIER FAMILY 25 MEMBER 48; Mitochondrial substrate carrier protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCATGCCTACTTGGTATGCAGGTATTCAACAGGACTGTGTATGCGT |
| Internal bar code: | CCGGGGACTGTAGATACGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCAGTGCTCCCCACCTA |
| Suggested primer 2: | CAAAACGTGTTTAGCCGGCA |