| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038657 |
| Chromosome: | chromosome 16 |
| Location: | 6084160 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677600 | SOM5 | S-adenosyl-L-methionine-dependent O-methyltransferase; (1 of 4) PTHR13600//PTHR13600:SF12 - LEUCINE CARBOXYL METHYLTRANSFERASE // LEUCINE CARBOXYL METHYLTRANSFERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCCGCACACGGAGTGTGTAGAATCTTCTTCCTCGACTGTTTACATA |
| Internal bar code: | TGGATCCTATTGTCGTTAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3205 |
| LEAP-Seq percent confirming: | 82.4324 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGCTGGTGAGATCTGC |
| Suggested primer 2: | CTTGGCGCTGGTGAAGTAGA |