Insertion junction: CLIP2.038662_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.038662
Chromosome:chromosome 13
Location:1638035
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573550 ANK26 Predicted protein with ankyrin repeats; (1 of 7) PF12796//PF13637 - Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCGGCAGCAGCAGCGGGAGCAGCAGCCAACGACGCATAAAAAGAGGG
Internal bar code:ACAAGCGTCCCTTCCTCCTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2218
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGAGTGGTCCAACATCCC
Suggested primer 2:GCTAGAGGCCTGGCTTAAGG