| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038670 |
| Chromosome: | chromosome 10 |
| Location: | 175474 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418900 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGATGTGGGGTGCGCCATGTGGGGTCGTTTAAAGGGAAGGCAGTGAG |
| Internal bar code: | GAATGCAGTCAAGAGCGAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCTGGTCGTCCATCTCG |
| Suggested primer 2: | AGAGAGCCTCGGATGTTCCT |