| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038675 |
| Chromosome: | chromosome 14 |
| Location: | 2730219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626000 | (1 of 6) PF03547 - Membrane transport protein (Mem_trans) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTTGCCCAGCCAGAGGCTGTAGCAGCTGCAGGGGCTGTAGCAACTG |
| Internal bar code: | CAGATCGGACGTTGGTTTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1302 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTGAAACACACAGGCG |
| Suggested primer 2: | GCAGTGCCACTATTGATGCG |