| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.038690 |
| Chromosome: | chromosome 3 |
| Location: | 4663154 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177100 | (1 of 10) PTHR31867:SF1 - EXPANSIN-A1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGTGGGATTGTGGCGAGCGGCGAGCGGCACGAACCGAGCAGCATGCC |
| Internal bar code: | GCAGGCTGTCGAGACATGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGTTTGTGTGTGCATG |
| Suggested primer 2: | CTAATGGCTGTGCGCATTCC |