| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038701 |
| Chromosome: | chromosome 17 |
| Location: | 2353211 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713800 | (1 of 17) PF04755 - PAP_fibrillin (PAP_fibrillin) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTGGAAAGGGCTTCCCAGGCCACGTCATAGTGGCACATGAAGCTATGG |
| Internal bar code: | GAGCTTGGATATTGTTTAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2371 |
| LEAP-Seq percent confirming: | 82.5 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCGACTCGCAATCGTATG |
| Suggested primer 2: | CTTCAATGCACCCTCCCCAT |