| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.038704 |
| Chromosome: | chromosome 12 |
| Location: | 4847903 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523600 | (1 of 3) PTHR13367//PTHR13367:SF8 - TUMOR NECROSIS FACTOR-RELATED // OTU DOMAIN-CONTAINING PROTEIN 7B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGCGGACGGGCGGCTCTACTCCATCAACATCCTGGACGGGACTGTG |
| Internal bar code: | GTGTCTGTGACAGTTTCGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1987 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGACGCTGAGGAAGGTA |
| Suggested primer 2: | TCGACAGCAACATCAGCACT |