Insertion junction: CLIP2.038713_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038713
Chromosome:chromosome 16
Location:1970145
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g656400 SQD1 (1 of 1) 3.13.1.1 - UDP-sulfoquinovose synthase / Sulfite:UDP-glucose sulfotransferase; UDP-sulfoquinovose synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGACTTGCTGAAGACCCAGCTCGTTAGCGCTGGTAGGCAGGCAGTGTG
Internal bar code:TAATCCGTGCCCTTACCTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1127
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTCTGTTGGGATGAGTGC
Suggested primer 2:GTGATTTCCACCCCGACACT