| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038737 |
| Chromosome: | chromosome 13 |
| Location: | 1122880 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569400 | TAUD1 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATTGCAACTGCAAATGGAACGCAATGCCCAGCCCCTGTGCCCCACC |
| Internal bar code: | GACGAGATTATATTATCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2702 |
| LEAP-Seq percent confirming: | 90.099 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 101 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCTGACTCGTGGTTCTT |
| Suggested primer 2: | ATTTTGCGGCGTGGAGTTTC |