Insertion junction: CLIP2.038739_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038739
Chromosome:chromosome 15
Location:1689803
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre19.g750897 (1 of 1) 3.5.4.12 - dCMP deaminase / Deoxycytidylate deaminase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCGCTCCGCCCATCTCGCGCACATACACACACACGCTCTGGCAGCCC
Internal bar code:GAGTCCGGCCACCAAAAAACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:934
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCCACTCACTTCACTTCT
Suggested primer 2:CACTGCTCCATCCTCCGAC