Insertion junction: CLIP2.038742_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.038742
Chromosome:chromosome 6
Location:7548162
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800736 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAGAGACAGCAGCAGACTGCTGTACTGCGACATGCTCAGCGCTGCTGC
Internal bar code:GGGTTTCATAACCGGCGGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:408
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAAGTGATGCGCAAGATG
Suggested primer 2:GGTTGGTAGCGGGAGTATCG