Insertion junction: CLIP2.038765_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.038765
Chromosome:chromosome 7
Location:4871251
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346317 GT90F39,GT90-39 GT90 family protein 39; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGAAAAGCGGTACAGGAGGGTGAGGTGGTTGAGGCTGAGGAAGGAAGT
Internal bar code:CCCTTAGAAGGGCGCACCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:952
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GACCATCCCACACACGTCAT
Suggested primer 2:CAGTAAGCAACCCTGGGAGG