| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038770 |
| Chromosome: | chromosome 3 |
| Location: | 7577351 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATTCCTCCCTTTCCCACATCCTTCCGCTCGCTCGATCGACAGGTCGA |
| Internal bar code: | TAGTTAGTCGGAACGAGTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1936 |
| LEAP-Seq percent confirming: | 95.6044 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACACACGACATGAGCT |
| Suggested primer 2: | AAGCTTCCAAGTCCCTCACG |