Insertion junction: CLIP2.038773_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038773
Chromosome:chromosome 16
Location:5370623
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683350 TEH6 Acyl-CoA thioesterase-like protein; (1 of 2) K17361 - acyl-coenzyme A thioesterase 9 [EC:3.1.2.-] (ACOT9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCCGCCAAAGATGACGTCGAAGGCGTTGCGGTCCTGGCTGCGGTGCG
Internal bar code:ATGCATTCCATGGGATTGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1946
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGATGCATAGGTAGGCG
Suggested primer 2:ATCGCTTACGCCCATGTAGG