Insertion junction: CLIP2.038775_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038775
Chromosome:chromosome 7
Location:6350258
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g357200 UGD1,EZY12 Similar to UDP-Glucose Dehydrogenase; (1 of 3) 1.1.1.22 - UDP-glucose 6-dehydrogenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGCTGTTCCAGGCCTTGATACGCTCCTCGTTGATGTCAACGACGACCA
Internal bar code:CCCTTCGATCGAGATGCATGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2064
LEAP-Seq percent confirming:88.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGCTTGTGAATTGGCG
Suggested primer 2:GGGGGTTTTGGGGGAGTATG