| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038821 |
| Chromosome: | chromosome 16 |
| Location: | 2396215 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659600 | (1 of 1) K14834 - nucleolar complex protein 3 (NOC3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGCTGGGGCTGGCTGCGGTGAGCTGGCTGTCGCTGTGGCTGTGCCT |
| Internal bar code: | TGTCTGAAAGAATCGATACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCATACAGCTGCACGT |
| Suggested primer 2: | TCCTCCTCCTCTGGTGATGG |