Insertion junction: CLIP2.038834_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038834
Chromosome:chromosome 12
Location:3433873
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g497653 SNE13,NED1 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Putative Cinnamoyl-CoA reductase/flavanone 4-reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCATCATGTATGGCCGCCACTCGCACTAGCTTTACTGTCCGCGATTTG
Internal bar code:AAGCGGACCAGATGACGGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:151
LEAP-Seq percent confirming:5.17241
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGGTCTGCGTCACTGGAG
Suggested primer 2:CGCACAGGAACAAGGGTTTG