| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038841 |
| Chromosome: | chromosome 12 |
| Location: | 7014036 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560650 | (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGGCTCCAGGTGGTCTGGTCGGCACGCACCGGCGCCTCCAGCAGCCC |
| Internal bar code: | GTTGTTCGAGGTAAGTCTCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5197 |
| LEAP-Seq percent confirming: | 73.5849 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACACTGGTGCGTCAAAT |
| Suggested primer 2: | CAAAACCGGGCGAACAATGT |