| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038850 |
| Chromosome: | chromosome 7 |
| Location: | 5602256 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352250 | HEL42 | (1 of 2) K14780 - ATP-dependent RNA helicase DHX37/DHR1 [EC:3.6.4.13] (DHX37, DHR1); DEAD/DEAH box helicase | intron |
| Cre07.g352251 | (1 of 1) PTHR18934//PTHR18934:SF99 - ATP-DEPENDENT RNA HELICASE // ATP-DEPENDENT RNA HELICASE DHX37-RELATED | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTGTATACATACCGCAATCATAGCAGATAGACTACTGCTGCGCATGC |
| Internal bar code: | GGGTTACTCATTTTTGTTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1775 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGCAGGCGTAATAAC |
| Suggested primer 2: | CGGGCCATTCTTATGCATGC |