| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038869 |
| Chromosome: | chromosome 7 |
| Location: | 1330074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322450 | HLM13 | putative N-methyltransferase; (1 of 1) PF00855//PF00856 - PWWP domain (PWWP) // SET domain (SET) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCTCGGGGTGCAGGTCCATGGTGCCGCTGCGAGGAGGAGGAGGAGGA |
| Internal bar code: | CACGGATCGAGCGGATAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGACACATCCTGCCTC |
| Suggested primer 2: | ACGTACAGCTCTTCACCTGC |