Insertion junction: CLIP2.038869_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038869
Chromosome:chromosome 7
Location:1330079
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g322450 HLM13 putative N-methyltransferase; (1 of 1) PF00855//PF00856 - PWWP domain (PWWP) // SET domain (SET) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGCGGCGCGACCTGGGCCGGCGCGTGGAGGTGTTCTGGGACGCGGACG
Internal bar code:CCAAAGTTTTTCAGAATTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:37.5
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTACAGCTCTTCACCTGC
Suggested primer 2:TTTGGGACACATCCTGCCTC