Insertion junction: CLIP2.038871_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038871
Chromosome:chromosome 12
Location:5226808
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g527400 PGM9 Phosphoglycerate mutase, 2%252C3-bisphosphoglycerate-independent; (1 of 2) 5.4.2.12 - Phosphoglycerate mutase (2,3-diphosphoglycerate-independent) / Phosphoglyceromutase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTGCGTCTCCAGCACCCCGCAAATCGCGTCTGAAAGCGCGTTCACCA
Internal bar code:TAATTGTGGTCATATTAAAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1082
LEAP-Seq percent confirming:88.2353
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACAAATCCACACTGCGC
Suggested primer 2:TCTGCCCAACCATCTTGTCC