| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.038879 |
| Chromosome: | chromosome 1 |
| Location: | 7453800 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800135 | (1 of 1) PTHR30217//PTHR30217:SF10 - UNCHARACTERIZED PROTEASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCATCCGGCCTCACCGCCGCCCCCGCGGATGTGCCTCGCAGCCGCGG |
| Internal bar code: | AGGCTAGTAGTGGTCGAATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACAGCACACGAATGC |
| Suggested primer 2: | ACGCTTCCTGCAGGAGTAAC |