Insertion junction: CLIP2.038888_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.038888
Chromosome:chromosome 14
Location:228574
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g609100 (1 of 6) PTHR13339//PTHR13339:SF0 - COP9 SIGNALOSOME COMPLEX SUBUNIT 8 // COP9 SIGNALOSOME COMPLEX SUBUNIT 8 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCACGCCATCCACACCACGCTGCGTGGCGCTTTCCCGGCGCTCGAGGA
Internal bar code:GTTGTTTCTAGCCTAACTATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3394
LEAP-Seq percent confirming:97.2973
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGGCTCTTCATCGCTGGC
Suggested primer 2:GGACTCGCTGAACCCTTGAA